What is?
Plant transformation vectors are they special vectors witch used in transformation to plants or production of transgenic plants known as plant transformation vectors. they vectors are binary vectors, plasmids, etc. witch obtain from Agrobacterium tumefaciens. that bacteria causing tumors in plants and a super vector to production to transgenic plants. commonly the genes to be transferred are cloned between the left and right T-DNA border of so called binary T-DNA vectors. that can be replicate in both E.coli and Agrobacterium tumefaciens.
Plasmids- All plasmids are a DNA molecule, can replicated independently in host cell. That occur in circular, double stranded, form. They have sixe from 1 kbp to 1000 kbp. They plasmids used i genetic engineering called plasmids vectors. They used as expression of desire gene in host cells and make transformation successful.
Binary vectors- it is composed of the border of T-DNA, cloning sites, replication function for bacteria likeAgrobacterium tumefaciens, and E.coli, markers genes, reporter genes, and other witch improved this efficiency. useful vectors are widely circulated. vectors with compatible selectable markers and convenient cloning sites are top criteria when inserting gene of approx 15 kbp in this.
PLANT TRANSFORMATION VECTORS
Advantages – They molecule involves in process no recombination take place process. binary vectors are high efficient for integration and expression. e.g. pCIT series, pGPTV (glucuronidase plant transformation vector) series, pCG series vectors etc.
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